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Immunoreactive TNF- and IL-6 were measured by Duoset enzyme-linked immunosorbent assay (ELISA) development kits (R&D systems, MN, USA) according to the manufacturer's instruction

Adults Most adults only require 7 or more hours of sleep each night

Building on this idea, Decottignies and colleagues used cybrids with mitochondrial DNA from one of the donors with average telomere length that showed the counterintuitive association with dysfunctional mitochondria
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