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Two weeks after lentivirus infusion, the offspring were used for behavior tests followed by biomedical analysis, as indicated in Mouse Protocol 1 ( DNA Methylation Analysis We developed a real-time PCR-based method for methylation-specific PCR (MSP) analysis on the human OXTR promoter according to the previously described method with some modifications ( 1 with the below details: Methylated primer: forward 5- ttt gag ttt att gtt aaa gtc gt -3, reverse 5- aaa taa taa tat tct tcc ccg aa -3

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Kolla BP, Schneekloth TD, Biernacka J, Shah V, Lazaridis KN, Geske J et al (2018) PNPLA3 association with alcoholic liver disease in a cohort of heavy drinkers