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Ferulic acid supplementation for six weeks at a dose of 1000 mg daily in a randomized, double-blind, placebo-controlled clinical trial resulted in significant reductions in total cholesterol, Low-Density Lipoprotein Cholesterol (LDL-C), triglycerides, and oxidative stress marker levels, followed by an increase in High-Density Lipoprotein Cholesterol (HDL-C)

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During the validation and optimization method, the internal standards dissolved in the four solvents were tested in a mixture of 100 % methanol, methanol/water (50:50) (v/v), methanol + 0.01 % acetic acid, and ammonium buffer pH 3.5 [H 2 O/ACN (90:10) (v/v) + 1 mM NH 4 (CH 3 COO)]
