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The histopathological hallmarks of ALD include steatosis, hepatocyte ballooning, and neutrophil infiltration (Stickel et al., 2017), mirrored biochemically by elevated serum transaminases, dyslipidemia, diminished antioxidant enzyme activities, and increased pro-inflammatory cytokines (Li et al., 2025)

10.1016/S0167-4889(97)00097-9 61 GioanniniT

Specimens were collected every ~48.75 hours, stored in 95% ethanol, and sorted to morphospecies

Using sterile technique, dilute the overnight starter culture 1:20 into 600 ml fresh LB supplemented with 100 g/ml ampicillin (see Note 11)
