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Description
The cells were then incubated with Alexa Fluor 488 dye-conjugated secondary antibody (1:150) at 37C for 1 h away from light
Juvenile and adult worms were carefully washed in phenol red free RPMI medium and subsequently incubated in phenol red free RPMI medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and at 37 C in a 5% CO 2 incubator overnight
doi: 10.1016/j.jcmgh.2022.01.007 162 DiBellaMThomasMSAlyousefHMillarCBlessoCMalyshevaOet al
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