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Figure 4 In summary, melatonin plays a pivotal role in regulating female reproductive hormone secretion and the menstrual cycle rhythm through its complex interactions with the hypothalamic-pituitary-ovarian (HPO) axisEnvironmental light exposure, patterns of melatonin secretion, and the dynamics of receptor expression collectively contribute to the maintenance of endocrine balance and physiological functions within the female reproductive system (78, 79)
To purify a protein with the help of their affinity to another molecule, the interacting partner (ligand), e.g., a protein, a small molecule, or a metal, is immobilized on the stationary phase of the chromatography matrix

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