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For gel electrophoresis, the immunoprecipitates were resuspended in reducing loading buffer (Novex, Life Technologies, Carlsbad, CA, USA) with TCEP (Pierce, Thermo Scientific, IL, USA) and the proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Life Technologies)

LCs were collected, washed twice with RPMI and resuspended in RPMI with 10% fetal bovine serum (10,270,106, Gibco) and 1 X Antibiotic-Antimycotic (15,240,062, Gibco)

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Moreover, the compound has been shown to precipitate a reduction in AMP-activated protein kinase activity and elevations in the levels of the apoptosis mediator - Fas ligand in the hippocampus and in the pro-apoptotic Bax protein (Sadek et al., 2016, Dief et al., 2014)