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When confluent, HAECs were shifted to ALI culture with 50 L apical media for 8 days to achieve full differentiation with and without IL-13 (10 ng/mL) stimulation (R&D Systems, 213-ILB)
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P21 resists ferroptosis in osteoarthritic chondrocytes by regulating GPX4 protein stability
There is no established upper tolerable limit, though adverse reactions such as hypersensitivity can occur rarely
