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Since NRF2 is a transcriptional activator, we hypothesize that these genes are rapidly activated by OS and ER stress induced by Cyss deprivation and are only indirectly regulated by NRF2 as it plays a role in balancing OS, mitochondrial dysfunction and also ER stress [39, 40] Of the 22 DEGs that were negatively regulated by Cyss deprivation and positively regulated by exogenously expressed NRF2, 19 remained upregulated in the presence of exogenously expressed NRF2 even in the absence of Cyss and consequently could be direct targets of NRF2 transcriptional activity and involved in the survival of Hs 578T cells under these conditions

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