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After 18 hours of treatment, cells were collected and resuspended in sterile PBS before undergoing the following staining with 10 M Liperfluo (Dojindo, #L-248-10) for 30 minutes at 37C

Repeated impingement and mechanical stress can cause collagen fibrils to undergo deformation, affecting their alignment and cross-linking patterns

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This review underscores the importance of ongoing interdisciplinary research to develop new, effective, and safe therapeutic options for thyroid diseases, with Scrophulariae Radix positioned as a promising candidate for future clinical integration