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Description
Briefly, the rabbit reticulocyte lysates (RRLs) containing the radiolabelled reporter protein (0.21.0 l RRL per import reaction) was diluted in 10 l import buffer (0.25 M sucrose, 50 mM KCl, 20 mM MOPS-KOH (pH 7.2), 5 mM MgCl 2 , 2 g ml 1 E-64 and 48 g ml 1 methionine, final concentration)
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