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However, the assay has the disadvantage that in comparison with the fluorescent spot test and the spectrophotometric assay, it is a time-consuming and technically difficult test

10.1186/s12933-019-0828-y 34 ChangS.-S.LeeV

Tominaga T, Murat ML, Dubourdieu D

These quality-control measures help reduce variability and support researchers working across long-term projects and experimental models requiring dependable sourcing