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The optical density (OD) values of each well at 24, 48, 72, and 96 h were measured at a wavelength of 450 nm using an Epoch2 microplate reader (BioTek, USA)
TIGAR relocates to mitochondria under hypoxia, forms a complex with hexokinase 2 (HK2) on the outer membrane of mitochondria, and regulates the activity of HK2, helping to control the ROS of mitochondria (Cheung et al., 2012)
& Bechara, E
doi: 10.1038/sj.bjc.6601611 65 KalluriRZeisbergM
