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This particular form represents the most reduced and chemically reactive state of cobalamin, rendering it highly receptive to binding a methyl group from a form of vitamin B9 called 5-methyltetrahydrofolate, the product of methylenetetrahydrofolate reductase (MTHFR), to generate methylcobalamin (MeCbl)

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In contrast, the 2,3-diamino substitution in the 1MQ scaffold (2,3-diamino-1MQ) displayed high passive permeability (Table 1), but moderate bi-directional active transport with moderate efflux ratio (Table 2)