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Hara KY, Kiriyama K, Inagaki A, Nakayama H, Kondo A (2012) Improvement of glutathione production by metabolic engineering the sulfate assimilation pathway of Saccharomyces cerevisiae

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coli DH5 culture (1 10 3 cells/ml) was added to the mixtures, further incubated for 15 min at 37C, and EDTA was added to a final concentration of 10 mM to stop the serum killing

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