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To verify the health status of the rats before and after administration, blood samples were collected at 0 and 24 h after administration of test substances and sent to the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University for blood tests to determine creatinine, alanine transaminase (ALT), and aspartate transaminase (AST) with normal range 0.20.7 mg/dL, 1356 U/L, and 34109 U/L, respectively
ViraniSSAlonsoABenjaminEJBittencourtMSCallawayCWCarsonAPet alHeart disease and stroke statistics2020 update: a report from the American Heart Association
Obviously our decision to follow these individuals may have had a profound influence on outcome