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HUVECs transiently transfected with LOX-PP and EV for 48 h were seeded with 25,000 cells/well in the upper chamber of the transwell culture insert with 1% FBS containing EBM-2
The monobromobimane adduct was monitored with a scanning fluorescence detector (Waters 2475) with an excitation wavelength of 370 nm and an emission wavelength of 485 nm
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