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The following thermocycler conditions were used, initial activation at 98C for 30 s and then subjected to 35 cycles of amplification (denaturation at 98C for 10 s, annealing at 58C for 30 s and elongation at 72C for 90 s) followed by a final elongation step at 72C for 2 min (BIOMETRA T3000)

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To determine the role of endogenously produced H 2 S on inflammatory organ injury, we examined the outcomes of d-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF in CSE-deficient mice on the C57BL6 background

Parnis FX, Coleman RE, Harper PG, et al