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To optimize drug design and develop therapies targeted at disease mechanisms involving the BBB or to improve drug delivery to the CNS, BBB models are required that adequately reflect in vivo conditions

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Similar content being viewed by others Introduction The liver is the largest endocrine organ in the body and is critical for maintaining homeostasis, serving as the primary site of xenobiotic metabolism, production of coagulation factors, and removal of ammonia as well as a multitude of other essential functions 1

Materials and Methods Animals Adult C57BL/6 male mice aged 68 weeks and weighing 2026 g were purchased from the Animal Center of the Chinese Academy of Sciences (Shanghai, China)