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Degraded peptide delivers less active compound

However, the therapeutic utility of doxorubicin is undermined by its dose-dependent cardiotoxicity, possibly arising as a result of futile redox cycling, with NADH dehydrogenase within the mitochondria proposed as the most likely site of anthracycline reduction 9,10

As you titrate on the same concentration: 4 mg = 0.80 mL = 80 units, 8 mg = 1.60 mL = 160 units (this exceeds a single 100-unit barrel, so it is drawn as two fills), and 12 mg = 2.40 mL = 240 units (drawn across multiple fills)

The idea is to build a clear routine, rather than simply adding redundant formulas