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These lead to oxidative and nitrosative stress, respectively, a condition where cellular structure and function are more damaged, thereby disrupting intracellular homeostasis

As the sensitization of HPA axis during the pubertal maturation might modulate stress reactivity and brain maturation in adolescents 33 , the dysregulated HPA activity in those late/post-pubertal adolescents with high family conflict might have far-reaching negative impacts on their mental and physical health development 14,33

Cells were extracted with 200 L of ice-cold 80% methanol, centrifuged at 15,000 g for 20 min at 4C and lyophilized

Hypoxia Inducible Factor 1 (HIF-1), a crucial transcriptional regulator of hypoxic responses, is upregulated under oxidative stress, which subsequently triggers a hypoxic response, enhances the transcription of angiogenic factors, such as vascular endothelial growth factor (VEGF), and mediates autophagy, mitophagy, and aerobic glycolysis [134]