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The cell pellet was resuspended in ice-cold 100 mM Tris, pH 7.6, centrifuged again, and finally resuspended in 1 mL of the same buffer

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Chemical and Biomolecular Strategies for STING Pathway Activation in Cancer Immunotherapy

Similar to WT albumin, both QMP variants demonstrated strict pH-dependent binding to FcRn, with rapid dissociation from the immobilized receptor upon injection of a pH 7.4 buffer (Supplementary Fig