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Alexa Fluor conjugated secondary antibodies were used to fluorescently label the antigens of interest and following dehydration in a series of increasing concentrations of methanol and cleared using dibenzyl ether, the membrane-bound slices were placed onto a glass microscope slide and imaged using a Zeiss LSM-800 inverted confocal microscope

For example, a small number of subjects reported the following mild side effects in the course of a four-week glutathione study at the dose of 1g/day [13]: Flatulence Loose stools Flushing Weight gain In a more specialized study involving cystic fibrosis patients and much higher glutathione doses, some of the patients reported tightness in the torso, diarrhea, and fever-like symptoms [14]

If your protocol calls for working concentrations in the low-micromolar range typical of cell-based assays, the stock will need serial dilution in your assay buffer