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To generate the initial anti-MBP IgG construct, total RNA was isolated from 4 10 7 hybridoma cells using a Qiagen RNeasy kit with the following modifications: 4 10 7 cells were resuspended in 600 l RLT buffer, homogenized with hand-held homogenizer for 2 min followed by the addition of 1,800 l RLT buffer (2,400 total, 600 l per 1 10 7 cells), mixed, separated into 4 600-l aliquots and homogenized for another 30 s

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