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For animal experiments, the dried extract was freshly dissolved in PBS immediately prior to use at a volume sufficient for 6 days of treatment (three injections, administered every other day)

Hhn TJ, Grune T

doi: 10.4274/tjod.galenos.2019.46244 79

Animal Model:Neonatal Sprague-Dawley rats (male, 18-22g, postnatal day 7), hypoxic-ischemic brain damage (HIBD) model [2] Dosage:100 mg/kg Administration:Intraperitoneal injection, once daily, for 14 consecutive days starting 24 hours after HIBD induction Result:Brain injury: Reduced infarct volume by 40% compared to the HIBD control group as measured by triphenyltetrazolium chloride (TTC) staining