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Some studies show saliva has much better repeatability for the onset of melatonin release (DLMO), e.g., 16 out of 17 people showed repeatable results
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We assume the value of E Cl to be such that Cl concentration is at equilibrium, that is the Cl synaptic influx and extrusion through KCC2 cancel each other out, this yields where V mean is the time averaged value the membrane potential taken to be V mean = 60 mV
