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Oral contraceptive use is associated with smaller hypothalamic and pituitary gland volumes in healthy women: A structural MRI study

Briefly, adult fat bodies were dissected in 1 phosphate buffered saline (PBS), fixed 20 min in 4% paraformaldehyde at room temperature (RT), rinsed 5 in 1 PBS, then permeabilized for 30 min at RT in 1xPBS + 0.3% Triton X-100
