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doi: 10.1016/j.aninu.2021.02.008

When 1 mM of the precursor was supplemented, 15.9 M L-carnitine could be found in the supernatant (1.6% molar yield as judged by the L-carnitine and TML concentrations in the culture medium)

The limit of quantification (LOQ) was defined as the lowest concentrations with the RSDs lower than 5% and accuracies within 5%, considering at least ten times the response compared to that of the blank

In addition, TMAO induces several inflammatory pathways, which cause the activation of pro-inflammatory cytokines such as IL-6 and TNF- which causes chronic inflammation thereby causing plaque formation and plaque rupture leading to cardiovascular disease [71]