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Acetaldehyde, a reactive metabolite, forms adducts with cellular proteins, nucleic acids, and lipids, disrupting cellular functions (Rocco, 2014)

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After tipping off the supernatant, diluted serum samples were incubated for 2 h at RT

ELISA-based protein quantification indicated that LPS stimulated secretion of proinflammatory proteins including TNF-, Ccl2, and IL-6 from BV-2 cells (Fig
