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Biochem J (2009) 417:113.10.1042/BJ20081386 36 KaminskiMMSauerSWKaminskiMOppSRuppertTGrigaraviciusPet alT cell activation is driven by an ADP-dependent glucokinase linking enhanced glycolysis with mitochondrial reactive oxygen species generation
For dose-response cytotoxicity experiments, use at least 810 compound concentrations spanning 34 logs to accurately determine IC50 values
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such activity levels can easily be determined with high precision using only a few microliters of whole blood and a simple spectrophotometric assay
