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Subsequently, they were subsectioned to 10 M slices using a Cryostat, placed on a charged microscopy slide and processed for TUNEL positivity

H.et al (2012)

In these individuals it would appear that whilst they were able to exist as relatively healthy individuals, often not knowing that they had methylation associated mutations, some initiating factor such as a prolonged infection, combined with low vitamin D levels resulted in gradual loss of their vitamin B12 supplies leading to the CFS symptomatology being apparent, and the subsequent discovery of their methylation status

Duration and Difficulty: This circuit lasts approximately 2.5 to 3.5 hours, depending on the visitors pace and the time spent exploring each area