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doi: 10.1016/j.ebiom.2020.102793 159 WagenblastESotoMGutirrez-ngelSHartlCAGableALMaceliARet al
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Leucine also contributes to cell growth and the formation of sterols
GST pull-down vs immunoprecipitation Preparing your glutathione beads Loading your GST-fusion protein onto the glutathione beads Adding the interacting proteins Eluting the GST-fusion and interacting proteins Materials needed for GST pull-down assays Preparing glutathione agarose beads Loading GST-fusion proteins onto glutathione beads GST pull-downs are assays used to investigate protein-protein interactions
