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2.14 Differential scanning calorimetry (DSC) Being a tool that provides a thermodynamic profile of protein unfolding/denaturation process by measuring the heat absorbed or released by a protein sample as its temperature is changed, differential scanning calorimetry (DSC) is different from the biophysical technique discussed so far, all of which provide information on some structural features of a protein, such as its secondary structure, flexibility, hydrodynamic dimensions, or solvent/protease accessibility