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Acta Histochem (2012) 114(8):82735
R.YamasakiR.et al
Recombinant N-terminally MRGS(H) 6 -tagged wild-type and mutant ScGrx7 and HsGrx5 C122S (without their N-terminal targeting sequences 19 ) were purified after lysozyme treatment and sonication by Ni-NTA affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl, and 50 mM sodium phosphate, pH 8.0 11,19,25,42
10.1128/JB.187.2.752-757.2005 J
