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Fluorescence intensity was read at excitation 550 nm, emission 600 nm for red fluorescence and at excitation 485 nm, emission 535 nm for green fluorescence using an M5E fluorescence plate reader

Sample Preparation Animals were fasted overnight and for clinical chemistry blood samples from each rat were collected from the eyes by retro-orbital puncture using blood capillary tubes without heparin as per requirement under mild ether anesthesia blood samples were incubated at room temperature for 10 minutes and left to clot then centrifuged at 3000 r.p.m for 10 min and the serum were collected, serum was separated and kept in clean stopper plastic vial at 80C until the analysis of serum parameters

L-Carnitina debe tomarse antes de la actividad fsica y notars que es absorbida por el cuerpo muy rpidamente

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