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1f), 50 nM of SPY555-FastAct (Cytoskeleton Spirochrome CY-SC205), 50 nM of SPY650-Tubulin (CY-SC503), and a MemGlow 488 (1:200,000) (Cytoskeleton MG01-10) were incubated with the neurons for 30 min prior to imaging

He specifically warned that LPS contamination can be cumulative it is not that one injection causes a reaction, but the inflammatory load builds until a tipping point is reached

doi: 10.1038/nrdp.2017.34

Using vented needles or introducing air displacement volume into the vial during withdrawal reduces the negative pressure that causes coring, extending both mechanical vial integrity and solution sterility