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For DBS and VAMS just a simple extraction step with organic solvent mixtures is needed prior to HPLC-MS/MS analysis but, depending on the blood volume sampled, lower analytical performance has to be taken into account (Osteresch et al

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Yet, in contrast to eed1 , efg1 / cph1 mutants are avirulent during systemic and catheter infection models, suggesting that some degree of morphology changes as in eed1 , or genes under control of efg1 / cph1 are required in vivo 7,48

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