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Biochemistry (Moscow)

Methods Cell culture and growth conditions All cell lines were cultured in either RPMI (Gibco, 11875119) or DMEM media (Gibco, 12430063) supplemented with 10% fetal bovine serum (Gibco, 12483020) and 1% penicillin-streptomycin (Gibco, 15140-122) and passaged by washing with Dulbeccos phosphate buffered saline (PBS) (Gibco, 14190250), and incubating with trypsin-EDTA (0.25%) (Gibco, 25200114)

While medications such as anti-inflammatories and corticosteroids can help manage symptoms, many people are seeking additional strategies that support long term joint health

First, research has shown that IF can lead to nutritional deficiencies due to reduced intake during fasting periods [53, 119]