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Freshly made paraformaldehyde stock solution was added to obtain a final concentration of 0.25%
Sivanand S, Vander Heiden MG (2020) Emerging roles for branched-chain amino acid metabolism in cancer

data-dependent MS 2 settings were as follows: resolution 17,500, automatic gain control target 2 10 5 , maximum injection time 100 ms, loop count 10, isolation window 2.0 m / z , normalized collision energy 28, charge state 1 and 7 excluded, dynamic exclusion of 15 s) on undeuterated samples

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