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What were looking at here is a signaling peptide that interacts with several overlapping repair and regeneration pathways, such as: Upregulation of vascular endothelial growth factor (VEGF), accelerating angiogenesis and new blood vessel formation Activation of the nitric oxide (NO) signaling pathway, driving vasodilation and tissue perfusion Modulation of growth hormone receptor expression, amplifying GH sensitivity at the tissue level Stimulation of tendon fibroblast proliferation and collagen synthesis Downregulation of inflammatory cytokines, including TNF-alpha and IL-6 Direct cytoprotective effects on the gastrointestinal mucosa In other words: A peptide with systemic effects across multiple tissues

The cell suspension was then distributed into a 48-well cell culture plate

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