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Assays were performed in triplicate for each sample on a Roche Lightcycler 480 system (Roche, Basel, Switzerland) using Realtime Ready DNA probes Mastermix supplemented with ResoLight Dye (Roche) according to the manufacturers protocol

Pinealon protocols Pinealon is available in both injectable and oral/sublingual formulations, giving researchers flexibility in administration

Singh, et al., 2022)

Under control conditions without EpoB, most injured axons degenerated or stalled (84% retracted, 12% stalled, n = 33), with no observed growth or regeneration within 1 h of axotomy
