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The primary antibodies used in the study included anti-AKT (1:1,000, Affinity Biosciences, Changzhou, China), anti-p-AKT (1:1,000, Cell Signaling Technology, Shanghai, China), anti-p38 MAP kinase (1:1,000, Affinity Biosciences), anti-phosphorylated p38 (1:1,000, Cell Signaling Technology), and anti--actin (1:2,000, Sangon, Shanghai, China)

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Moreover, deacetylase classes of HDAC4 and HDAC2 levels were significantly upregulated in areas of the hippocampus, prefrontal cortex, and the basal forebrain region of neuronal cultured cells, in AD mouse models, (Giusti-Rodrguez et al., 2011)