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Briefly, cells are cultured with the STEMdiff TM definitive endoderm basal medium with supplements A and B for 24h followed by STEMdiff TM definitive endoderm basal medium with supplement B for 48h with daily medium change (Xia et al., 2017)

Sneaking ligand construct (SLC1) is a ligand fusion protein that effectively inhibits the NF-B signaling pathway and transcription of proinflammatory cytokines after selective endocytosis [438]

After the imaging, the cells were trypsinised, re-suspended in the complete media, and relative number of living cells were counted by using Countess II after staining with trypan blue

10.1152/jn.1997.78.1.335 J