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Springer, Singapore (2024)
Whether purified STAND-A36 recognised mouse Syt I/II in the brain was investigated by isolating mouse brains and homogenising them in a buffer composed of 20 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 0.5% TritonX-100, and a complete protease inhibitor cocktail (Roche) by shearing with a 23-G, then with a 27-G, needle syringe and agitating for 1 h at 4 C
Water is the enemy of peptide stability
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