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Nonetheless, higher lipid levels in the liver and muscle, as well as increased mass of visceral adipose tissue, are frequently identified in most fish when fed with HFD 107,108

Medical marijuana is currently legal in 30 states, including the District of Columbia, and recreational marijuana is legal in a handful of states, most recently California (as of January 1, 2018)

Immunofluorescence and microscopy Frozen brain tissue sections (8 m thickness) in glass slides were washed with PBS, fixed in acetone-methanol (11 v/v) fixative, blocked the cellular antigen with 3% bovine serum albumin at room temperature for 1 hr in the presence of 0.4% Triton X-100 and incubated with respective primary antibodies such as mouse anti-cPT1/ant-cPT2 (1250 dilution) and rabbit anti-neurofilament (1250 dilution) for overnight at 4C

Kitzman, N