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Gibson et al., 2019)
Without adequate antioxidant protection, the mitochondria cannot recover, and the patient remains trapped in a state of profound energy depletion
Lisbon, Portugal: European Monitoring Centre for Drugs and Drug Addiction
Fixation was carried out by incubating the cells with 4% paraformaldehyde in PBS containing 0.1% saponin for 45 min at 4 C, followed by washing with PBS supplemented with 0.1% saponin and 1% BSA (SBP)
