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Exclusion criteria taking dietary or multivitamin supplements containing vitamin B 12

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For hair growth, the relevant signaling pathways are: vascular stimulation (more blood flow to the dermal papilla), follicle repair (extending the anagen growth phase), inflammation modulation (calming the chronic low-grade inflammation that contributes to follicular miniaturization), and systemic growth signaling (IGF-1 levels that influence overall regenerative capacity)

The plate was incubated at room temperature for 510 min, before luminescence was measured using a Victor 3 1420 Multilabel Counter (Wallac 1420 Workstation, version 3.00 revision 5