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Following a PBS rinse, 10 g/ml DAPI (Invitrogen, Carlsbad, CA) in PBS was applied to sections for 5 min

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ATP production analysis The ENLITEN ATP Assay System (Promega, Madison, WI, USA) was used according to the manufacturers instructions

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It is to note that zonulin belongs to a family of structurally and functionally related proteins named zonulin family peptides that can affect intestinal permeability

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Animal models demonstrated improved neurological outcomes in Parkinsons disease

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