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Ca 2+ acts as a key second messenger in regulating various physiological changes during capacitation, including hyperactivation of motility (Snchez-Crdenas et al., 2018), membrane fluidity alterations, and the activation of signaling cascades essential for the acrosome reaction ( 2+ concentrations modulate the activity of Ca 2+ -sensitive enzymes ( 2+ is essential for ensuring successful fertilization which in turn could provide a more functional environment to drive preimplantation development past the maternal contribution

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