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Santonen T, Mahiout S, Alvito P et al (2023) How to use human biomonitoring in chemical risk assessment: methodological aspects, recommendations, and lessons learned from HBM4EU

To determine acivicin toxicity normal human dermal fibroblasts were treated with a range of concentrations of acivicin for 24 or 48 h and apoptosis was measured by Caspase-Glo 3/7 Assay System (Promega)

The epitopes are well known in literature and were identified by homolog-scanning mutagenesis, where segments of sequences from a homologous molecule (GAD 67) known not to bind to the specific antibodies are systematically substituted throughout the GAD 65 gene [57,58]

It was also found that the Michaelis constant (Km = 1.2 10 5 M) of FSP1 was relatively low, and the maximum reaction rate (Vmax = 4.1 10 7 M s 1) was much higher than that of related oxidoreductases, indicating that efficiency of FSP1